Anti-phospho-mTOR (Thr2164)

Analysis Note

ControlUnmodified and phosphorylated mTOR peptides

Application

Research CategorySignaling

Western Blot Analysis: A representative lot was used by an independent laboratory in HEK293 cell lysate. (Ekim, B., et al. (2011). MCB. [Epub ahead of print].)

Anti-mTOR Antibody is an antibody against phospho-mTOR (Thr2164) for use in Dot Blot, Western Blotting.

Research Sub CategoryKinases & Phosphatases

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The mammalian target of rapamycin (mTOR), also known as FRAP, RAFT, or RAPT, is a ubiquitously expressed serine/threonine kinase that belongs to the phosphoinositide 3-kinase–related kinase (PIKK) family. mTOR functions as a downstream effector in the PI3K pathway and is activated by the Akt kinase. mTOR responds to signals from growth factors and promotes protein synthesis underlying cell proliferation, by activation of the S6 kinase and inhibition of the 4E-BP protein involved in protein translation. Several studies have suggested that the proliferative functions of mTOR may contribute to the development of cancer.

Immunogen

Epitope: Phosphorylated Thr2164

KLH-conjugated linear peptide corresponding to human mTOR phosphorylated at Thr2164.

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Quality

Dot Blot Specificity Analysis: Unmodified and phosphorylated mTOR peptides were probed with Anti-phospho-mTOR (Thr2164) (1:1,000 dilution).

Specificity

This antibody recognizes mTOR phosphorylated at Thr2164.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

289 kDa calculated